abstract：BACKGROUND:Cardiovascular mortality is markedly higher in Russia than in Western Europe and the US. Little is known about indicators of atherosclerotic risk in the Russian population. To our knowledge, this is the first study of apolipoprotein (apo) AI and B in Russia based on the WHO-IFCC standard. METHODS:We measure...

journal_title：Clinical chemistry

authors： Averina M,Nilssen O,Brenn T,Brox J,Arkhipovsky VL,Kalinin AG

更新日期：2004-02-01 00:00:00

abstract：BACKGROUND:Our previously developed assay for detection of the factor V Leiden mutation (G1691A) based on a nucleic acid photo-cross-linking technology used two allele-specific capture probes and six fluorescein-modified signal-generating reporter probes. We wished to improve the sensitivity and performance of the meth...

journal_title：Clinical chemistry

authors： French C,Li C,Strom C,Sun W,Van Atta R,Gonzalez B,Wood M

更新日期：2004-02-01 00:00:00

abstract：BACKGROUND:Determinations of certain forms of prostate-specific antigen (PSA) have been shown to increase the specificity for prostate cancer (PCa). One such variant, proteolytically active PSA, is a potentially useful tumor marker, but it is not specifically recognized by antibodies. Using phage display libraries, we ...

journal_title：Clinical chemistry

authors： Wu P,Zhu L,Stenman UH,Leinonen J

更新日期：2004-01-01 00:00:00

abstract：BACKGROUND:Testing for troponin has important clinical value for patients who present with typical symptoms of acute coronary syndromes (ACS) such as chest pain (CP). Much less is known about the value of troponin testing for patients who present with other symptoms of ACS (anginal equivalent symptoms). METHODS:The ut...

journal_title：Clinical chemistry

authors： Ritter D,Lee PA,Taylor JF,Hsu L,Cohen JD,Chung HD,Virgo KS

更新日期：2004-01-01 00:00:00

abstract：BACKGROUND:Test repetition could be a readily modifiable component of laboratory utilization. Laboratory test repetition has not been rigorously studied at a population-based level. Our objective was to determine the prevalence of, and charges associated with, repetition of eight common laboratory tests. METHODS:We pe...

journal_title：Clinical chemistry

authors： van Walraven C,Raymond M

更新日期：2003-12-01 00:00:00

abstract：:It has been estimated that approximately 37% of the US population judged to be at high risk for developing coronary artery disease (CAD), based on the National Cholesterol Education Program guidelines, have increased plasma lipoprotein(a) [Lp(a)], whereas Lp(a) is increased in only 14% of those judged to be at low ris...

journal_title：Clinical chemistry

authors： Marcovina SM,Koschinsky ML,Albers JJ,Skarlatos S

更新日期：2003-11-01 00:00:00

abstract：BACKGROUND:There is need for a reference method for transferrin glycoforms in serum to which routine immunologic methods for the alcohol marker carbohydrate-deficient transferrin (CDT) can be traceable. We describe an improved HPLC method for transferrin glycoforms. METHODS:Transferrin was iron-saturated by mixing the...

journal_title：Clinical chemistry

authors： Helander A,Husa A,Jeppsson JO

更新日期：2003-11-01 00:00:00

abstract：BACKGROUND:Laboratory tests can be an important source of blood loss in hospitals, especially for newborns and patients in intensive care. The aim of this study was to quantify blood loss for laboratory diagnostic tests in a large number of patients in a teaching hospital. METHODS:We estimated blood loss by multiplyin...

journal_title：Clinical chemistry

authors： Wisser D,van Ackern K,Knoll E,Wisser H,Bertsch T

更新日期：2003-10-01 00:00:00

abstract：BACKGROUND:Paraoxonase may protect lipoproteins and cell membranes from peroxidation, and alterations in the activity of this enzyme have been associated with some chronic diseases. Serum paraoxonase appears to be mainly under genetic control, but some studies suggest that environmental factors may also modulate its ac...

journal_title：Clinical chemistry

authors： Ferré N,Camps J,Fernández-Ballart J,Arija V,Murphy MM,Ceruelo S,Biarnés E,Vilella E,Tous M,Joven J

更新日期：2003-09-01 00:00:00

abstract：BACKGROUND:C-reactive protein (CRP) is a widely recognized indicator of inflammation and is known to play an important role in atherogenesis. Recent prospective studies have demonstrated that increased CRP concentrations within the reference interval are a strong predictor of myocardial infarction, stroke, sudden cardi...

journal_title：Clinical chemistry

authors： Ledue TB,Rifai N

更新日期：2003-08-01 00:00:00

abstract：BACKGROUND:The aims of this study were to measure the N-terminal fragment of pro-brain natriuretic peptide (proBNP) in plasma in medical conditions commonly found in primary care and to evaluate the utility of these measurements in identifying impaired cardiac function in elderly patients with symptoms associated with ...

journal_title：Clinical chemistry

authors： Alehagen U,Lindstedt G,Eriksson H,Dahlström U

更新日期：2003-08-01 00:00:00

abstract：BACKGROUND:Two mutations in HFE, G845A (amino acid substitution C282Y) and C187G (H63D), are associated with hereditary hemochromatosis. We developed and validated a novel method, linked linear amplification (LLA), for detection of these two mutations. METHODS:Two segments of HFE were amplified by a multiplex LLA reac...

journal_title：Clinical chemistry

authors： Killeen AA,Breneman JW 3rd,Carillo AR,Ugozzoli LA,Lowery JD,Liu J,Hixson CS

更新日期：2003-07-01 00:00:00

abstract：BACKGROUND:D-2-Hydroxyglutaric aciduria (D-2-HGA), L-2-hydroxyglutaric aciduria (L-2-HGA), and the combined D/L-2-hydroxyglutaric aciduria (D/L-2-HGA) are poorly understood organic acidurias. To investigate the usefulness of cultured human skin fibroblasts for both diagnostic and research purposes, we measured disease-...

journal_title：Clinical chemistry

authors： Struys EA,Verhoeven NM,Roos B,Jakobs C

更新日期：2003-07-01 00:00:00

abstract：BACKGROUND:Fecal calprotectin (FC) has been proposed as a marker of inflammatory bowel disease (IBD), but few studies have evaluated its usefulness in patients with chronic diarrhea of various causes. We evaluated the diagnostic accuracy of a FC assay in identifying "organic" causes of chronic diarrhea in consecutive a...

journal_title：Clinical chemistry

authors： Carroccio A,Iacono G,Cottone M,Di Prima L,Cartabellotta F,Cavataio F,Scalici C,Montalto G,Di Fede G,Rini G,Notarbartolo A,Averna MR

更新日期：2003-06-01 00:00:00

abstract：BACKGROUND:The TDx Fetal Lung Maturity II (FLM II) assay uses amniotic fluid to assess lung maturity of the unborn infant. We investigated common preanalytical factors that influence FLM results, including centrifugation, sample storage, and contamination by whole blood. METHODS:We tested 18 specimens after centrifuga...

journal_title：Clinical chemistry

authors： Grenache DG,Parvin CA,Gronowski AM

更新日期：2003-06-01 00:00:00

abstract：BACKGROUND:External quality assurance (EQA) programs for diagnostic tests based on nucleic acid amplification have not been widely implemented in clinical laboratories and remain limited to few tests. Development of specific EQA programs based on application-based proficiency testing for any diagnostic molecular target...

journal_title：Clinical chemistry

authors： Raggi CC,Pinzani P,Paradiso A,Pazzagli M,Orlando C

更新日期：2003-05-01 00:00:00

abstract：BACKGROUND:Clinical diagnoses were correlated with results of a Co(II)-albumin binding assay in 167 patients treated at an emergency department of a health maintenance organization. METHODS:Patients were evaluated as being nonischemic or potentially ischemic through standard coronary disease indicators [creatine kinas...

journal_title：Clinical chemistry

authors： Bhagavan NV,Lai EM,Rios PA,Yang J,Ortega-Lopez AM,Shinoda H,Honda SA,Rios CN,Sugiyama CE,Ha CE

更新日期：2003-04-01 00:00:00

abstract：BACKGROUND:Endothelial nitric oxide synthase (eNOS) could be a candidate gene for coronary artery disease (CAD). This study investigated the relationship of the eNOS Glu(298)-->Asp and T(786)-->C polymorphisms with the presence and severity of CAD in the Italian population. METHODS:We enrolled 415 unrelated individual...

journal_title：Clinical chemistry

authors： Colombo MG,Paradossi U,Andreassi MG,Botto N,Manfredi S,Masetti S,Biagini A,Clerico A

更新日期：2003-03-01 00:00:00

abstract：BACKGROUND:Uremic syndrome is the consequence of the retention of solutes usually cleared by the healthy kidneys. p-Cresol can be considered a prototypic protein-bound uremic toxin. It is conceivable, analogous with drugs, that the non-protein-bound fraction of p-cresol exerts toxicity. This aspect had never been evalu...

journal_title：Clinical chemistry

authors： De Smet R,Van Kaer J,Van Vlem B,De Cubber A,Brunet P,Lameire N,Vanholder R

更新日期：2003-03-01 00:00:00

abstract：BACKGROUND:Previous methods to measure glycohemoglobin (GHb) have been time-consuming or imprecise; we therefore developed a new enzymatic assay for GHb. METHODS:Blood cells were first hemolyzed, and hemoglobin was digested with protease to yield fructosyl amino acid. Fructosyl amino acid oxidase acts on the fructosyl...

journal_title：Clinical chemistry

authors： Sakurabayashi I,Watano T,Yonehara S,Ishimaru K,Hirai K,Komori T,Yagi M

更新日期：2003-02-01 00:00:00

abstract：BACKGROUND:An abnormal IgLkappa:IgLlambda ratio has long been used as a clinical criterion for non-Hodgkin B-cell lymphomas. As a first step toward a quantitative real-time PCR-based multimarker diagnostic analysis of lymphomas, we have developed a method for determination of IgLkappa:IgLlambda ratio in clinical sample...

journal_title：Clinical chemistry

authors： Ståhlberg A,Aman P,Ridell B,Mostad P,Kubista M

更新日期：2003-01-01 00:00:00

abstract：BACKGROUND:Some of the genetic variants of hemoglobin (Hb) and their chemically modified species are known to affect the measurement of Hb A(1c). The purpose of this study was to characterize Hb species in the blood sample of a 74-year-old German male with an exceptionally low Hb A(1c) value. METHODS:Hemolysates from ...

journal_title：Clinical chemistry

authors： Bissé E,Schauber C,Zorn N,Epting T,Eigel A,Van Dorsselaer A,Wieland H,Kister J,Kiger L

更新日期：2003-01-01 00:00:00

abstract：BACKGROUND:The genotype of hepatitis C virus (HCV) is a predictor of antiviral therapeutic response. We describe an approach for HCV genotype determination by real-time PCR and melting curve analysis. METHODS:After automated nucleic acid extraction, we used reverse transcription-PCR in a block cycler to amplify nucleo...

journal_title：Clinical chemistry

authors： Bullock GC,Bruns DE,Haverstick DM

更新日期：2002-12-01 00:00:00

abstract：BACKGROUND:Because cyclosporin A (CsA) and glucocorticoids inhibit the production of interleukin-2 (IL-2) and other cytokines, quantitative analysis of cytokine mRNA might constitute a pharmacodynamic measure for immunosuppressive drug effects. We investigated whether immunosuppressive drugs influence cytokine mRNA exp...

journal_title：Clinical chemistry

authors： Härtel C,Fricke L,Schumacher N,Kirchner H,Müller-Steinhardt M

更新日期：2002-12-01 00:00:00

abstract：BACKGROUND:The analytical and clinical performance of homogeneous LDL-cholesterol assays has been reported, but their reactions with subfractions of LDL and VLDL have not been described in detail. METHODS:We evaluated reaction selectivity of two homogeneous LDL-cholesterol assays, LDLk (Kyowa Medex) and LDLd (Daiichi ...

journal_title：Clinical chemistry

authors： Usui S,Kakuuchi H,Okamoto M,Mizukami Y,Okazaki M

更新日期：2002-11-01 00:00:00

abstract：BACKGROUND:The management of critically ill infants and neonates includes frequent determination of arterial blood gas, electrolyte, and hematocrit values. An objective of attached point-of-care patient monitoring is to provide clinically relevant data without the adverse consequences associated with serial phlebotomy....

journal_title：Clinical chemistry

authors： Billman GF,Hughes AB,Dudell GG,Waldman E,Adcock LM,Hall DM,Orsini EN Jr,Koska AJ,Van Marter LJ,Finer NN,Kulhavy JC,Feld RD,Widness JA

更新日期：2002-11-01 00:00:00

abstract：BACKGROUND:To allow multianalyte binding assays, we have developed a novel polystyrene microtiter plate containing 24 main wells, each divided into 7 subwells. We explored its clinical potential by developing a PCR-chemiluminescent immunoassay (PCR-CLEIA) for simultaneous detection and typing of seven high oncogenic ri...

journal_title：Clinical chemistry

authors： Roda A,Mirasoli M,Venturoli S,Cricca M,Bonvicini F,Baraldini M,Pasini P,Zerbini M,Musiani M

更新日期：2002-10-01 00:00:00

abstract：BACKGROUND:Rapid intraoperative parathyroid hormone (PTH) measurements are an important prerequisite for minimally invasive parathyroidectomy, serving as a feasible marker for "cure" because of the short half-life of PTH. Because automated analysis may facilitate monitoring, two automated PTH assays were compared with ...

journal_title：Clinical chemistry

authors： Bieglmayer C,Prager G,Niederle B

更新日期：2002-10-01 00:00:00

abstract：BACKGROUND:The detection of monoclonal free light chains (FLCs) is an important diagnostic aid for a variety of monoclonal gammopathies and is especially important in light-chain diseases, such as light-chain myeloma, primary systemic amyloidosis, and light-chain-deposition disease. These diseases are more prevalent in...

journal_title：Clinical chemistry

authors： Katzmann JA,Clark RJ,Abraham RS,Bryant S,Lymp JF,Bradwell AR,Kyle RA

更新日期：2002-09-01 00:00:00

abstract：BACKGROUND:The Dutch project "Calibration 2000" aims at harmonization of laboratory results via calibration by development of matrix-based secondary reference materials. We considered the selection, preparation, and characterization of 34 potential reference materials (PRMs). METHODS:Sixteen PRMs were prepared either ...

journal_title：Clinical chemistry

authors： Cobbaert C,Weykamp C,Baadenhuijsen H,Kuypers A,Lindemans J,Jansen R

更新日期：2002-09-01 00:00:00

abstract：BACKGROUND:Advances in the biological sciences and technology are providing molecular targets for diagnosing and treating cancer. Current classifications in surgical pathology for staging malignancies are based primarily on anatomic features (e.g., tumor-node-metastasis) and histopathology (e.g., grade). Microarrays to...

journal_title：Clinical chemistry

authors： Bernard PS,Wittwer CT

更新日期：2002-08-01 00:00:00

abstract：BACKGROUND:KLK5 is a newly discovered human kallikrein gene. Many kallikrein genes have been found to be differentially expressed in various malignancies, and prostate-specific antigen (PSA; encoded by the KLK3 gene) is the best tumor marker for prostate cancer. Like the genes that encode PSA and other kallikreins, the...

journal_title：Clinical chemistry

authors： Yousef GM,Scorilas A,Kyriakopoulou LG,Rendl L,Diamandis M,Ponzone R,Biglia N,Giai M,Roagna R,Sismondi P,Diamandis EP

更新日期：2002-08-01 00:00:00

abstract：BACKGROUND:In malignancy, gene mutations frequently occur in tumor suppressor genes such as p53 and are sporadically located. We describe a homogeneous method for amplification and mutation scanning, and apply the method to the p53 gene. METHODS:Using a series of overlapping fluorescein-labeled oligonucleotides comple...

journal_title：Clinical chemistry

authors： Millward H,Samowitz W,Wittwer CT,Bernard PS

更新日期：2002-08-01 00:00:00

abstract：BACKGROUND:At concentrations <500 microg/L, serum alpha-fetoprotein (AFP) has low specificity in the diagnosis of hepatocellular carcinoma (HCC), but monosialylated AFP (msAFP) is more specific for HCC. We describe two strategies for quantitative analysis of msAFP and explore their diagnostic accuracy in cases of HCC w...

journal_title：Clinical chemistry

authors： Poon TC,Mok TS,Chan AT,Chan CM,Leong V,Tsui SH,Leung TW,Wong HT,Ho SK,Johnson PJ

更新日期：2002-07-01 00:00:00

abstract：BACKGROUND:Plasma creatinine concentration and calculated creatinine clearance are of limited value as glomerular filtration rate (GFR) markers in patients with decompensated liver cirrhosis. We assessed plasma cystatin C as an indicator of GFR in such patients. METHODS:We studied 36 patients with decompensated liver ...

journal_title：Clinical chemistry

authors： Orlando R,Mussap M,Plebani M,Piccoli P,De Martin S,Floreani M,Padrini R,Palatini P

更新日期：2002-06-01 00:00:00

abstract：BACKGROUND:Because reference values for vitamin B(12) concentrations and vitamin B(12)-binding capacities for pregnant women have not been established, the reference values for nonpregnant women are often applied to assess vitamin B(12) status. The aim of the present study was to describe ranges of biochemical indices ...

journal_title：Clinical chemistry

authors： Koebnick C,Heins UA,Dagnelie PC,Wickramasinghe SN,Ratnayaka ID,Hothorn T,Pfahlberg AB,Hoffmann I,Lindemans J,Leitzmann C

更新日期：2002-06-01 00:00:00

abstract：BACKGROUND:Remnant lipoprotein-cholesterol (RLP-C) concentrations in sera of healthy individuals are very low (0.080-0.437 mmol/L), making conventional cholesterol methods poorly suited to this purpose. We have developed a highly sensitive cholesterol assay (CD method) and applied it to the RLP-C assay. METHODS:The CD...

journal_title：Clinical chemistry

authors： Kishi K,Ochiai K,Ohta Y,Uemura Y,Kanatani K,Nakajima K,Nakamura M

更新日期：2002-05-01 00:00:00

abstract：BACKGROUND:The purpose of this study was to assess the feasibility of infrared (IR) spectroscopy for the simultaneous quantification of serum LDL-cholesterol (LDL-C) and HDL-cholesterol (HDL-C) concentrations. METHODS:Serum samples (n = 90) were obtained. Duplicate aliquots (5 microL) of the serum specimens were dried...

journal_title：Clinical chemistry

authors： Liu KZ,Shaw RA,Man A,Dembinski TC,Mantsch HH

更新日期：2002-03-01 00:00:00

abstract：BACKGROUND:The "unit" for "enzymic activity" (U = 1 micromol/min) was recommended by the International Union of Biochemistry and Molecular Biology (IUB) in 1961 and is widely used in medical laboratory reports. The general trend in metrology, however, is toward global standardization through defining units coherent wit...

journal_title：Clinical chemistry

authors： Dybkaer R

更新日期：2002-03-01 00:00:00

abstract：BACKGROUND:Apolipoprotein (apo) E is a component of two major classes of plasma lipoproteins, apo B- (apo E-LpB) and non-apo B-containing (apo E-Lp-non-B) lipoproteins. The factors that affect total apo E in particles [lipoprotein E (LpE), apo E-Lp-non-B, and apo E-LpB], are incompletely characterized. METHODS:We stud...

journal_title：Clinical chemistry

authors： Schiele F,Vincent-Viry M,Starck M,Beaud B,Hennache G,Siest G,Visvikis S,Herbeth B

更新日期：2002-02-01 00:00:00